Chapter 4: DNA Lab
Introduction: In these labs, we will be testing to find a solution for DNA isolation. This lab consists of 4 different labs. Each of the different labs has us doing a different activity involving the process of finding a DNA solution.
Materials:
Analytical balance
Tabeltop milligram Balance
Lab scoops
Sodium Chloride
15mL Capped tubes
Tube racks
Edta
TRIS
Disodium Salt
125 ml bottle
Glasses
Graduated Cylinder
Sodium Hydroxide
Hydrochloric acid
Glass Rods
50 ml beakers
2 ml pipette
Salmon sperm DNA
P1000 micropipette
Ethanol, 95%
Permanent markers
1ml plastic peaker
TAE buffer concentrate, 40X
600ml beaker
Agarose
Microwave
Hand protecters, heat
Gel box
Water bath
Rea
ction tubes, 1.5ml
DNA samples
Loading dye
Micro-pipettes
Media bottle, 250ml
Microcentrifuge
Ethidium Bromide
Gloves
UV light
Lab 4a
Purpose: The purpose of this lab was to make 10ml of 5ml NaCl. Also, to make 100ml of 10mm Tris and 1mm EDTA(DNA storage solution).
Procedure:
1. Prepare solution of 0.8% agarose in 1X TAE buffer solution.
2. Weigh out required powdered agarose in a weigh boat. Place in 250-mL media bottle.
3. Measure out TAE to prepare 100 mL of agarose and buffer mixed together.
4. Dissolve agarose in microwave by bringing to boil.
5. Let cool until cool enough to hold.
6.Pour the gel into a gel box.
7. Put comb into the notches in the gel tray.
8. Pour 1X TAE buffer onto the gel box until covering the gel.
9.Wrap in Saran wrap and store.
Lab 4j
Purpose:
Materials:
Analytical balance
Tabeltop milligram Balance
Lab scoops
Sodium Chloride
15mL Capped tubes
Tube racks
Edta
TRIS
Disodium Salt
125 ml bottle
Glasses
Graduated Cylinder
Sodium Hydroxide
Hydrochloric acid
Glass Rods
50 ml beakers
2 ml pipette
Salmon sperm DNA
P1000 micropipette
Ethanol, 95%
Permanent markers
1ml plastic peaker
TAE buffer concentrate, 40X
600ml beaker
Agarose
Microwave
Hand protecters, heat
Gel box
Water bath
Rea
ction tubes, 1.5ml
DNA samples
Loading dye
Micro-pipettes
Media bottle, 250ml
Microcentrifuge
Ethidium Bromide
Gloves
UV light
Lab 4a
Purpose: The purpose of this lab was to make 10ml of 5ml NaCl. Also, to make 100ml of 10mm Tris and 1mm EDTA(DNA storage solution).
Procedure:
1. Prepare solution of 0.8% agarose in 1X TAE buffer solution.
2. Weigh out required powdered agarose in a weigh boat. Place in 250-mL media bottle.
3. Measure out TAE to prepare 100 mL of agarose and buffer mixed together.
4. Dissolve agarose in microwave by bringing to boil.
5. Let cool until cool enough to hold.
6.Pour the gel into a gel box.
7. Put comb into the notches in the gel tray.
8. Pour 1X TAE buffer onto the gel box until covering the gel.
9.Wrap in Saran wrap and store.
Lab 4j
Purpose: